Rapid Protocol, High Transformation Efficiency, and Stable after a Freeze-Thaw Cycle.
JetGiga DH5α DynaCompetent Cells
JetGiga DH5α DynaCompetent Cells are chemically competent E.coli cells which perform high transformation efficiency.
Highlights
- High transformation efficiency：＞1×109 CFU/μg (pUC19)
- Rapid 6-minute protocol.
- Maintain a high transformation efficiency after a single aliquot (1×109 CFU/μg).
Performance Data
Comparison with Competitor Products (described as rapid transformation)
Strong Resistance to Freeze-thaw Cycles
After once or twice freeze-thaw cycle, JetGiga DH5α shows high transformation efficiency.
Protocol
6-minute Protocol with Higher Transformation Efficiency (＞1×109 CFU/μg)
(1) Thaw JetGiga DH5α on ice.
(2) Add plasmid DNA to the cells and mix gently by tapping.
(3) Incubate the cells on ice for 5 minutes.
(4) Incubate the cells in a 42℃ water bath for 30 seconds.
(5) Spread it on selection plates.
10-minute Heat-Shock-Free Protocol with High Transformation Efficiency (＞1×108 CFU/μg)
This protocol is suitable for automation due to the absence of a heat-shock step.
(1) Thaw JetGiga DH5α on ice.
(2) Add plasmid DNA to the cells and mix gently by tapping.
(3) Incubate the cells on ice for 5 minutes.
(4) Add Recovery Medium to the tube and vortex it for 1 second.
(5) Incubate the cells at room temperature for 5 minutes.
(6) Spread it on selection plates.
Citations
Kenyu I., et al., Cell Reports Methods, 3(8):100542 (2023).
Shigetoshi K., et al., Nucleic Acids Research, 51(4):e24 (2023).
EN
BDL is a member of the Funakoshi Group.
